Nitrolite | Nitrolite Avidin
Nitrolite
encompasses the advantage of reversibility of biotin-binding and the
absence of non-specific binding which may represent a real plus in
affinity chromatography.
The selective nitration of tyrosine residues in three of the four biotin-binding sites of Neutralite avidin considerably reduces the affinity of the protein for biotinylated molecules above pH 9. Consequently, biotinylated probes can be adsorbed at neutral pH and released at pH ~10. Free biotin blocks the remaining high-affinity biotin-binding sites that have not been nitrated. Nitroavidin is particularly useful for separating and purifying biotin conjugates from complex mixtures. Nitroavidin also provides a regenerable capture reagent for functionalization of surface plasmon resonance (SPR) immunosensors2. In its neutral/deglycosylated form (NitroLite Avidin), it encompasses the advantage of reversibility of biotin-binding and the absence of nonspecific binding which may represent a real plus in affinity chromatography. The biotin-binding capacity of Nitroavidin derivatives is typically min 10 µg free biotin per mg protein.
Nitrolite Avidin is a chemically & enzymatically modified avidin characterized by a neutral isoelectric point and the absence of surface glycans. NeutraLite Avidin is carbohydrate-free and neutral, with the double advantage of the presence of a large number of amino acids available for derivatization, and the absence of RYD sequences. Altogether, these molecular characteristics yield the lowest nonspecific binding among known biotin-binding proteins, yet a specific activity near the theoretical maximum of approximately 14 μg/mg of protein
The selective nitration of tyrosine residues in three of the four biotin-binding sites of Neutralite avidin considerably reduces the affinity of the protein for biotinylated molecules above pH 9. Consequently, biotinylated probes can be adsorbed at neutral pH and released at pH ~10. Free biotin blocks the remaining high-affinity biotin-binding sites that have not been nitrated. Nitroavidin is particularly useful for separating and purifying biotin conjugates from complex mixtures. Nitroavidin also provides a regenerable capture reagent for functionalization of surface plasmon resonance (SPR) immunosensors2. In its neutral/deglycosylated form (NitroLite Avidin), it encompasses the advantage of reversibility of biotin-binding and the absence of nonspecific binding which may represent a real plus in affinity chromatography. The biotin-binding capacity of Nitroavidin derivatives is typically min 10 µg free biotin per mg protein.
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Nitrolite | Nitrolite Avidin |
Nitrolite Avidin is a chemically & enzymatically modified avidin characterized by a neutral isoelectric point and the absence of surface glycans. NeutraLite Avidin is carbohydrate-free and neutral, with the double advantage of the presence of a large number of amino acids available for derivatization, and the absence of RYD sequences. Altogether, these molecular characteristics yield the lowest nonspecific binding among known biotin-binding proteins, yet a specific activity near the theoretical maximum of approximately 14 μg/mg of protein
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